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How to apply an Intraperitoneal Injections (I.P) to mice

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Restrain the mouse by the scruff method. Expose the ventral side of the animal, tilting the head down at a slight angle.

Prep the site with 70% ethanol.

The sterile needle should be placed, bevel up, in the lower right or left quadrant of the animal’s abdomen.

Insert the needle at a 30° angle.

Aspirate to ensure proper placement and inject the material.